Journal: Nature Communications

Article Title: DNA synthesis for true random number generation

doi: 10.1038/s41467-020-19757-y

Figure Lengend Snippet: Major procedural steps in synthesizing random DNA strands during solid state DNA synthesis. DNA building blocks are mixed prior to entering the binding substrate, where they start forming a strand of DNA based on their coupling efficiencies. The rate of the individual nucleotides couplings, r i , can be approximated by multiplication of the respective rate constant, k i and the nucleotide concentration, c i . During the process, individual nucleotides are shielded from binding to other nucleotides using protecting groups, ensuring that only one new random nucleotide can bind per DNA strand per iteration. Excess nucleotides that have not found a DNA strand to bind to are then removed from the synthesis chamber, and DNA strands are de-protected. To elongate each DNA strand to the desired length, the process of adding a mix of nucleotides, washing off left-over and subsequently de-protecting is repeated as often as required. Once the desired strand length of DNA has been reached, the DNA is cleaved from the synthesis support.

Article Snippet: Random DNA sequences as illustrated in Fig. were synthesized commercially three times: twice by Microsynth and once by Eurofins Genomics.

Techniques: DNA Synthesis, Binding Assay, Concentration Assay

Journal: Nature Communications

Article Title: DNA synthesis for true random number generation

doi: 10.1038/s41467-020-19757-y

Figure Lengend Snippet: Commercial DNA synthesis of random nucleotides from a Microsynth (synthesis 1), b Microsynth (synthesis 2), c Eurofins Genomics. d Heat map showing normalized nucleotide pair distribution along the strand length of Microsynth 1 synthesis (Fig. 3a). For the three syntheses, illustrated are the 60 nucleotides of the random section of DNA strands, respectively, based on the design featured in Fig. . The direction of synthesis is indicated by arrows. Analyzed sample size ca. 700,000 sequences each. e The mechanism of G–T transversion can be a source for the effect of percentage variation of G and T along the strand of DNA (position nonequivalence). Source data are provided in the Source Data file.

Article Snippet: Random DNA sequences as illustrated in Fig. were synthesized commercially three times: twice by Microsynth and once by Eurofins Genomics.

Techniques: DNA Synthesis